Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

Emilie Montellier; Fayçal Boussouar; Sophie Rousseaux; Kai Zhang; Thierry Buchou; François Fenaille; Hitoshi Shiota; Alexandra Debernardi; Patrick Héry; Sandrine Curtet; Mahya Jamshidikia; Sophie Barral; Hélène Holota; Aurélie Bergon; Fabrice Lopez; Philippe Guardiola; Karin Pernet; Jean Imbert; Carlo Petosa; Minjia Tan; Yingming Zhao; Matthieu Gérard; Saadi Khochbin

Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

Emilie Montellier ¹ Fayçal Boussouar Sophie Rousseaux ¹ Kai Zhang Thierry Buchou ¹ François Fenaille Hitoshi Shiota Alexandra Debernardi ¹ Patrick Héry Sandrine Curtet ¹ Mahya Jamshidikia Sophie Barral ¹ Hélène Holota Aurélie Bergon Fabrice Lopez Philippe Guardiola Karin Pernet Jean Imbert Carlo Petosa ² Minjia Tan Yingming Zhao Matthieu Gérard Saadi Khochbin ¹

1 CNRS UMR 5309, INSERM U1209

2 IBS - UMR 5075 - Institut de biologie structurale

Abstract : The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.

Type de document :

Article dans une revue

Genes & development, 2013, 27 (15), pp.1680-92

Domaine :

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire / Biologie structurale [q-bio.BM]

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http://hal.univ-grenoble-alpes.fr/hal-01322388
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Soumis le : vendredi 27 mai 2016 - 10:17:46
Dernière modification le : lundi 11 février 2019 - 16:50:12

Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

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Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

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