MALDI-ToF mass spectrometry for studying noncovalent complexes of biomolecules.

Abstract : Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been demonstrated to be a valuable tool to investigate noncovalent interactions of biomolecules. The direct detection of noncovalent assemblies is often more troublesome than with electrospray ionization. Using dedicated sample preparation techniques and carefully optimized instrumental parameters, a number of biomolecule assemblies were successfully analyzed. For complexes dissociating under MALDI conditions, covalent stabilization with chemical cross-linking is a suitable alternative. Indirect methods allow the detection of noncovalent assemblies by monitoring the fading of binding partners or altered H/D exchange patterns.
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Article dans une revue
Topics in current chemistry, Springer, 2013, 331, pp.1-36
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http://hal.univ-grenoble-alpes.fr/hal-01322386
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Soumis le : vendredi 27 mai 2016 - 10:14:59
Dernière modification le : jeudi 11 janvier 2018 - 06:15:24

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  • HAL Id : hal-01322386, version 1
  • PUBMED : 22371170

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Stefanie Mädler, Elisabetta Boeri Erba, Renato Zenobi. MALDI-ToF mass spectrometry for studying noncovalent complexes of biomolecules.. Topics in current chemistry, Springer, 2013, 331, pp.1-36. 〈hal-01322386〉

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