BEST-TROSY experiments for time-efficient sequential resonance assignment of large disordered proteins

Abstract : The characterization of the conformational properties of intrinsically disordered proteins (IDPs), and their interaction modes with physiological partners has recently become a major research topic for understanding biological function on the molecular level. Although multidimensional NMR spectroscopy is the technique of choice for the study of IDPs at atomic resolution, the intrinsically low resolution, and the large peak intensity variations often observed in NMR spectra of IDPs call for resolution- and sensitivity-optimized pulse schemes. We present here a set of amide proton-detected 3D BEST-TROSY correlation experiments that yield the required sensitivity and spectral resolution for time-efficient sequential resonance assignment of large IDPs. In addition, we introduce two proline-edited 2D experiments that allow unambiguous identification of residues adjacent to proline that is one of the most abundant amino acids in IDPs. The performance of these experiments, and the advantages of BEST-TROSY pulse schemes are discussed and illustrated for two IDPs of similar length (~270 residues) but with different conformational sampling properties.
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Journal of Biomolecular NMR, Springer Verlag, 2013, 55 (4), pp.311-321. 〈10.1007/s10858-013-9715-0〉
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http://hal.univ-grenoble-alpes.fr/hal-01321583
Contributeur : Frank Thomas <>
Soumis le : jeudi 26 mai 2016 - 09:10:42
Dernière modification le : lundi 19 février 2018 - 14:34:03

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Zsófia Sólyom, Melanie Schwarten, Leonhard Geist, Robert Konrat, Dieter Willbold, et al.. BEST-TROSY experiments for time-efficient sequential resonance assignment of large disordered proteins. Journal of Biomolecular NMR, Springer Verlag, 2013, 55 (4), pp.311-321. 〈10.1007/s10858-013-9715-0〉. 〈hal-01321583〉

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