An introduction to biological NMR spectroscopy.

Abstract : NMR spectroscopy is a powerful tool for biologists interested in the structure, dynamics, and interactions of biological macromolecules. This review aims at presenting in an accessible manner the requirements and limitations of this technique. As an introduction, the history of NMR will highlight how the method evolved from physics to chemistry and finally to biology over several decades. We then introduce the NMR spectral parameters used in structural biology, namely the chemical shift, the J-coupling, nuclear Overhauser effects, and residual dipolar couplings. Resonance assignment, the required step for any further NMR study, bears a resemblance to jigsaw puzzle strategy. The NMR spectral parameters are then converted into angle and distances and used as input using restrained molecular dynamics to compute a bundle of structures. When interpreting a NMR-derived structure, the biologist has to judge its quality on the basis of the statistics provided. When the 3D structure is a priori known by other means, the molecular interaction with a partner can be mapped by NMR: information on the binding interface as well as on kinetic and thermodynamic constants can be gathered. NMR is suitable to monitor, over a wide range of frequencies, protein fluctuations that play a crucial role in their biological function. In the last section of this review, intrinsically disordered proteins, which have escaped the attention of classical structural biology, are discussed in the perspective of NMR, one of the rare available techniques able to describe structural ensembles. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 16 MCP).
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Article dans une revue
Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2013, 12 (11), pp.3006-25
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Soumis le : jeudi 26 mai 2016 - 09:04:49
Dernière modification le : mardi 3 juillet 2018 - 09:38:10

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Dominique Marion. An introduction to biological NMR spectroscopy.. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2013, 12 (11), pp.3006-25. 〈hal-01321578〉



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