Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains.

Abstract : We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.
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Article dans une revue
PLoS ONE, Public Library of Science, 2013, 8 (5), pp.e62947
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http://hal.univ-grenoble-alpes.fr/hal-01321573
Contributeur : Frank Thomas <>
Soumis le : jeudi 26 mai 2016 - 08:55:06
Dernière modification le : jeudi 11 janvier 2018 - 06:15:24

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Linnéa Isaksson, Maxim Mayzel, Maria Saline, Anders Pedersen, Joakim Rosenlöw, et al.. Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains.. PLoS ONE, Public Library of Science, 2013, 8 (5), pp.e62947. 〈hal-01321573〉

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