In Vivo Quantification of 5-HT2A Brain Receptors in Mdr1a KO Rats with 123I-R91150 Single-Photon Emission Computed Tomography.

Noé Dumas 1 Marcelle Moulin-Sallanon Pascal Fender 2 Benjamin B Tournier 1 Nathalie Ginovart Yves Charnay 1 Philippe Millet 3
1 HUG - Hôpitaux Universitaires de Genève
2 IBS - UMR 5075 - Institut de biologie structurale
3 Thales Research and Technology [Palaiseau]
Abstract : Our goal was to identify suitable image quantification methods to image 5-hydroxytryptamine2A (5-HT2A) receptors in vivo in Mdr1a knockout (KO) rats (i.e., P-glycoprotein KO) using 123I-R91150 single-photon emission computed tomography (SPECT). The 123I-R91150 binding parameters estimated with different reference tissue models (simplified reference tissue model [SRTM], Logan reference tissue model, and tissue ratio [TR] method) were compared to the estimates obtained with a comprehensive three-tissue/seven-parameter (3T/7k)-based model. The SRTM and Logan reference tissue model estimates of 5-HT2A receptor (5-HT2AR) nondisplaceable binding potential (BPND) correlated well with the absolute receptor density measured with the 3T/7k gold standard (r > .89). Quantification of 5-HT2AR using the Logan reference tissue model required at least 90 minutes of scanning, whereas the SRTM required at least 110 minutes. The TR method estimates were also highly correlated to the 5-HT2AR density (r > .91) and only required a single 20-minute scan between 100 and 120 minutes postinjection. However, a systematic overestimation of the BPND values was observed. The Logan reference tissue method is more convenient than the SRTM for the quantification of 5-HT2AR in Mdr1a KO rats using 123I-R91150 SPECT. The TR method is an interesting and simple alternative, despite its bias, as it still provides a valid index of 5-HT2AR density.
Type de document :
Article dans une revue
Molecular Imaging, Decker Publishing, 2015, 14
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http://hal.univ-grenoble-alpes.fr/hal-01219727
Contributeur : Frank Thomas <>
Soumis le : vendredi 23 octobre 2015 - 10:37:58
Dernière modification le : lundi 19 février 2018 - 14:34:03

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  • HAL Id : hal-01219727, version 1
  • PUBMED : 26105563

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Noé Dumas, Marcelle Moulin-Sallanon, Pascal Fender, Benjamin B Tournier, Nathalie Ginovart, et al.. In Vivo Quantification of 5-HT2A Brain Receptors in Mdr1a KO Rats with 123I-R91150 Single-Photon Emission Computed Tomography.. Molecular Imaging, Decker Publishing, 2015, 14. 〈hal-01219727〉

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