Structure determination of feline calicivirus virus-like particles in the context of a pseudo-octahedral arrangement.

Abstract : The vesivirus feline calicivirus (FCV) is a positive strand RNA virus encapsidated by an icosahedral T=3 shell formed by the viral VP1 protein. Upon its expression in the insect cell - baculovirus system in the context of vaccine development, two types of virus-like particles (VLPs) were formed, a majority built of 60 subunits (T=1) and a minority probably built of 180 subunits (T=3). The structure of the small particles was determined by x-ray crystallography at 0.8 nm resolution helped by cryo-electron microscopy in order to understand their formation. Cubic crystals belonged to space group P213. Their self-rotation function showed the presence of an octahedral pseudo-symmetry similar to the one described previously by Agerbandje and co-workers for human parvovirus VLPs. The crystal structure could be solved starting from the published VP1 structure in the context of the T=3 viral capsid. In contrast to viral capsids, where the capsomers are interlocked by the exchange of the N-terminal arm (NTA) domain, this domain is disordered in the T=1 capsid of the VLPs. Furthermore it is prone to proteolytic cleavage. The relative orientation of P (protrusion) and S (shell) domains is alerted so as to fit VP1 to the smaller T=1 particle whereas the intermolecular contacts around 2-fold, 3-fold and 5-fold axes are conserved. By consequence the surface of the VLP is very similar compared to the viral capsid and suggests a similar antigenicity. The knowledge of the structure of the VLPs will help to improve their stability, in respect to a use for vaccination.
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PLoS ONE, Public Library of Science, 2015, 10 (3), pp.e0119289
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http://hal.univ-grenoble-alpes.fr/hal-01162635
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Soumis le : jeudi 11 juin 2015 - 09:37:31
Dernière modification le : lundi 19 février 2018 - 14:34:03

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Wim P Burmeister, Marlyse Buisson, Leandro F Estrozi, Guy Schoehn, Olivier Billet, et al.. Structure determination of feline calicivirus virus-like particles in the context of a pseudo-octahedral arrangement.. PLoS ONE, Public Library of Science, 2015, 10 (3), pp.e0119289. 〈hal-01162635〉

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