Analytical Ultracentrifugation and Size-Exclusion Chromatography Coupled with Light Scattering for the Characterization of Membrane Proteins in Solution

Abstract : Analytical ultracentrifugation (AUC) sedimentation velocity (SV) with absorbance and interference detection and size-exclusion chromatography coupled with static and dynamic light scattering, absorbance, and refractive index detections (SEC/MALS) are two techniques that combine separation and analysis, in an absolute manner, of the mass and size of macromolecules in solution. We present here how they can be applied to the study of membrane proteins. We describe briefly the principles of the species separation, what the detection systems used measures, some theoretical background, the steps for data analysis with the example of the outer membrane protein FhuA, and emphasize the complementarity between these two techniques.
Type de document :
Chapitre d'ouvrage
Isabelle Mus-Veteau. Membrane Proteins Production for Structural Analysis, Springer, pp.267-287, 2014, 〈http://link.springer.com/〉
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http://hal.univ-grenoble-alpes.fr/hal-01160877
Contributeur : Frank Thomas <>
Soumis le : lundi 8 juin 2015 - 11:15:47
Dernière modification le : jeudi 11 janvier 2018 - 06:15:24

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  • HAL Id : hal-01160877, version 1

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Aline Le Roy, Cécile Breyton, Christine Ebel. Analytical Ultracentrifugation and Size-Exclusion Chromatography Coupled with Light Scattering for the Characterization of Membrane Proteins in Solution. Isabelle Mus-Veteau. Membrane Proteins Production for Structural Analysis, Springer, pp.267-287, 2014, 〈http://link.springer.com/〉. 〈hal-01160877〉

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