Mumps outbreak and laboratory diagnosis.

Abstract : Several mumps outbreaks have been reported in Europe and in the United States among highly vaccinated populations. Biological diagnosis is classically based on the detection of mumps-specific IgM, but the ability of serological tests to confirm mumps infection seems to be limited among vaccinated patients. We aim to report a mumps outbreak in an engineering school in Grenoble, France, from February to June 2013 and results of the biological testing. WHO definitions were used to define cases. Mumps--specific IgM and IgG were assessed by a commercially available EIA. Mumps RNA detection by real time reverse transcriptase polymerase chain reaction tests (RT-PCR) and mumps genotyping were performed by the French National Reference Centre for Paramyxoviridae. Sixty two mumps patient-cases were identified using WHO case definitions, 20 being biologically explored, of which 17 were confirmed by biological tests. Vaccination status was documented for 27 patients/62: 4 (14.8%) patients had received one dose of MMR vaccine, and 23 (85.2) two doses of MMR vaccine. Among the biologically explored patients, 83% had a positive RT PCR at the first sampling whereas only 45% had positive or equivocal IgM. All the genotyped strains were genotype G. Mumps laboratory diagnosis in a highly vaccinated population is challenging. Serological tests among vaccinated patients should be interpreted cautiously and confirmed by RT-PCR tests at the beginning of a mumps outbreak.
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Article dans une revue
Journal of Clinical Virology, Elsevier, 2014, 62, pp.14-9
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http://hal.univ-grenoble-alpes.fr/hal-01132342
Contributeur : Frank Thomas <>
Soumis le : mardi 17 mars 2015 - 09:30:17
Dernière modification le : mardi 22 mai 2018 - 20:40:08

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  • HAL Id : hal-01132342, version 1
  • PUBMED : 25542464

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Mylène Maillet, Eric Bouvat, Nicole Robert, Monique Baccard-Longère, Christine Morel-Baccard, et al.. Mumps outbreak and laboratory diagnosis.. Journal of Clinical Virology, Elsevier, 2014, 62, pp.14-9. 〈hal-01132342〉

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