Structural basis of eukaryotic cell-cell fusion.

Abstract : Cell-cell fusion proteins are essential in development. Here we show that the C. elegans cell-cell fusion protein EFF-1 is structurally homologous to viral class II fusion proteins. The 2.6 Å crystal structure of the EFF-1 trimer displays the same 3D fold and quaternary conformation of postfusion class II viral fusion proteins, although it lacks a nonpolar "fusion loop," indicating that it does not insert into the target membrane. EFF-1 was previously shown to be required in both cells for fusion, and we show that blocking EFF-1 trimerization blocks the fusion reaction. Together, these data suggest that whereas membrane fusion driven by viral proteins entails leveraging of a nonpolar loop, EFF-1-driven fusion of cells entails trans-trimerization such that transmembrane segments anchored in the two opposing membranes are brought into contact at the tip of the EFF-1 trimer to then, analogous to SNARE-mediated vesicle fusion, zip the two membranes into one.
Type de document :
Article dans une revue
Cell, Elsevier, 2014, 157 (2), pp.407-19
Liste complète des métadonnées

http://hal.univ-grenoble-alpes.fr/hal-01132332
Contributeur : Frank Thomas <>
Soumis le : mardi 17 mars 2015 - 09:12:34
Dernière modification le : jeudi 11 janvier 2018 - 06:26:03

Identifiants

  • HAL Id : hal-01132332, version 1
  • PUBMED : 24725407

Collections

Citation

Jimena Pérez-Vargas, Thomas Krey, Clari Valansi, Ori Avinoam, Ahmed Haouz, et al.. Structural basis of eukaryotic cell-cell fusion.. Cell, Elsevier, 2014, 157 (2), pp.407-19. 〈hal-01132332〉

Partager

Métriques

Consultations de la notice

105