Protein crystal structure obtained at 2.9 Å resolution from injecting bacterial cells into an X-ray free-electron laser beam.

Abstract : It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time (∼ 5 µs) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-Å-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information.
Type de document :
Article dans une revue
Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2014, 111 (35), pp.12769-74
Liste complète des métadonnées

http://hal.univ-grenoble-alpes.fr/hal-01131830
Contributeur : Frank Thomas <>
Soumis le : lundi 16 mars 2015 - 11:01:26
Dernière modification le : lundi 19 février 2018 - 14:34:04

Lien texte intégral

Identifiants

Collections

Citation

Michael R Sawaya, Duilio Cascio, Mari Gingery, Jose Rodriguez, Lukasz Goldschmidt, et al.. Protein crystal structure obtained at 2.9 Å resolution from injecting bacterial cells into an X-ray free-electron laser beam.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2014, 111 (35), pp.12769-74. 〈hal-01131830〉

Partager

Métriques

Consultations de la notice

122