Structural basis of pilus anchoring by the ancillary pilin RrgC of Streptococcus pneumoniae.

Abstract : Pili are surface-attached, fibrous virulence factors that play key roles in the pathogenesis process of a number of bacterial agents. Streptococcus pneumoniae is a causative agent of pneumonia and meningitis, and the appearance of drug-resistance organisms has made its treatment challenging, especially in developing countries. Pneumococcus-expressed pili are composed of three structural proteins: RrgB, which forms the polymerized backbone, RrgA, the tip-associated adhesin, and RrgC, which presumably associates the pilus with the bacterial cell wall. Despite the fact that the structures of both RrgA and RrgB were known previously, structural information for RrgC was still lacking, impeding the analysis of a complete model of pilus architecture. Here, we report the structure of RrgC to 1.85 Å and reveal that it is a three-domain molecule stabilized by two intradomain isopeptide bonds. RrgC does not depend on pilus-specific sortases to become attached to the cell wall; instead, it binds the preformed pilus to the peptidoglycan by employing the catalytic activity of SrtA. A comprehensive model of the type 1 pilus from S. pneumoniae is also presented.
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Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2014, 289 (24), pp.16988-97
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Soumis le : lundi 26 janvier 2015 - 11:38:34
Dernière modification le : lundi 19 février 2018 - 14:34:03

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Md Munan Shaik, Amandine Maccagni, Guillaume Tourcier, Anne-Marie Di Guilmi, Andréa Dessen. Structural basis of pilus anchoring by the ancillary pilin RrgC of Streptococcus pneumoniae.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2014, 289 (24), pp.16988-97. 〈hal-01109391〉

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