Novel Mn-SOD Mimetics Offer Superior Protection Against Oxidative Damages in Hek293 Kidney Cells
Résumé
A series of ternary Mn (II) complexes have been synthesized employing combination of thiosemicarbazones as primary
ligand and bipyridyl/aminoethanethiol as ancillary ligands and structurally characterized. The compounds were examined
for their protective effects in Xanthine–Xanthine oxidase-induced oxidative stress in HEK-293 kidney cells using MTT
assay, confocal imaging and Western blots. All compounds offer protection against the oxidative stress generated by
Xanthine–Xanthine oxidase system, while compounds having aminoethanethiol as ancillary ligands especially exhibit
potent effects even at nanomolar concentrations. These compounds seem to operate mechanistically through quenching
of ROS and enhancing Mn-SOD expression in mitochondria.